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Chromapure Licensing Crack
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1. The transcriptional control of the smooth muscle actin gene by steroid hormone receptors has been studied in chick heart fibroblast primary cultures. 2. The nuclease S1 protection assay has been used to detect nuclear actin mRNA as well as to assess the effect on transcription of three potential cis-acting regulatory elements located within 600 bp 5′ of the translation initiation codon. 3. Transfection experiments using fusion plasmids containing various deletion mutants of the smooth muscle actin gene showed that the sequence 5′-GCGAC-3′ essential for the induction of smooth muscle actin gene transcription by the steroid hormone receptor is also located within the octameric sequence TTACACGTGGAGTCCTGACGGGGCAGACCCAGGCCCAGCTCCCTCGGGGCAGACCCACGGGGCCAGACCCATGACGCCTGGTGGTGGTGGTGCCTTGGAAGAAGAGGACAGCGTGGAGATCTGGTGGCTGGAGCGGCAGG-3′. 4. These findings suggest that a complex of 3 steroid hormone receptors may be involved in smooth muscle actin gene transcription.Preserved cerebral blood
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